After laying out the contents of kit and priming all syringes, using the green filtered needle, draw up 5mL of Sodium Citrate (anticoagulant) into a 60mL syringe
Attach the 19G butterfly to the 60mL syringe containing the anticoagulant and draw 54mL of whole blood from the patient at a rate of 1mL per second, filling the syringe to 59mL.
Transfer the anticoagulated whole blood into the first Concentrating Device, containing the red cap and filter, at 1mL per second.
Fill the counterbalance with the equivalent volume of water and weigh on scale within ±3 grams. Place both devices in the centrifuge at opposite ends of the rotor and process.
Transfer the Platelet-Plasma Suspension (PPS) into the second Concentrating Device.
After the second spin we will see two layers: the Platelet-Poor Plasma (PPP) and the buffy-coat.
This is not Pure PRP yet.
Aspirate most of the Platelet-Poor Plasma (PPP) using the 30mL syringe leaving the volume you wish to inject in the concentrating device.
*This PPP will be used to micro-needle or as a BioFiller.*
The addition of Sodium Bicarbonate (10% of your final PRP volume) will counteract/buffer the acidic pH of the sodium citrate. This will make the procedure more comfortable for the patient.
Begin gently swirling the Concentrating Device to re-suspend the buffy-coat into your PPP making it rich in platelets. Continue doing so until the buffy-coat is completely re-suspended and there are no “chunks” in your now PRP.
Tilt the Concentrating Device to immerse the aspirating pipe into the PRP and draw up into the syringe(s) that will be used to inject.
It is recommended to use a 1mL luer-lock syringe with a 30G ½-inch needle to inject.
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