Training

Processing Using GS60 Pure PRP Kit

STEP 1

After laying out the contents of kit and priming all syringes, using the green filtered needle, draw up 5mL of Sodium Citrate (anticoagulant) into a 60mL syringe

STEP 2

Attach the 19G butterfly to the 60mL syringe containing the anticoagulant and draw 54mL of whole blood from the patient at a rate of 1mL per second, filling the syringe to 59mL.

STEP 3

Transfer the anticoagulated whole blood into the first Concentrating Device, containing the red cap and filter, at 1mL per second.

STEP 4

Fill the counterbalance with the equivalent volume
of water and weigh on scale within ±3 grams. Place
both devices in the centrifuge at opposite ends of the
rotor and process.

EXECUTIVE SERIES CENTRIFUGE SETTING

2 Minutes | 3800 RPM

PLATINUM SERIES CENTRIFUGE

Pre-Programmed Setting PurePRP® SP Spin 1

STEP 5

After the first spin, we will see the separation of our Platelet-Plasma Suspension (PPS) on top and the RBCs on the bottom. Using a 60mL syringe, aspirate the PPS until there is a full flash of red in the tubing.

STEP 6

Transfer the Platelet-Plasma Suspension (PPS) into the second Concentrating Device.

STEP 7

Fill the counterbalance with the equivalent volume of water and weigh on scale within ±3 grams. Place both devices in the centrifuge at opposite ends of the rotor and process.

EXECUTIVE SERIES CENTRIFUGE SETTING

5 Minutes | 3800 RPM

PLATINUM SERIES CENTRIFUGE

Pre-Programmed Setting PurePRP® SP Spin 2

STEP 8

After the second spin we will see two layers: the Platelet-Poor Plasma (PPP) and the buffy-coat.

This is not Pure PRP yet.

STEP 9

Aspirate most of the Platelet-Poor Plasma (PPP) using the 30mL syringe leaving the volume you wish to inject in the concentrating device.

*This PPP will be used to micro-needle or as a BioFiller.*

OPTIONAL STEP

RECOMMENDED STEP

The addition of Sodium Bicarbonate (10% of your final PRP volume) will counteract/buffer the acidic pH of the sodium citrate. This will make the procedure more comfortable for the patient.

STEP 10

Begin gently swirling the Concentrating Device to re-suspend the buffy-coat into your PPP making it rich in platelets. Continue doing so until the buffy-coat is completely re-suspended and there are no “chunks” in your now PRP.

STEP 11

Tilt the Concentrating Device to immerse the aspirating pipe into the PRP and draw up into the syringe(s) that will be used to inject.

It is recommended to use a 1mL luer-lock syringe with a 30G ½-inch needle to inject.

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